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Cotton bacterial blight (CBB), caused by the pathogenXanthomonas citrisubsp.malvacearum(Xcm), can inflict significant damage to cotton (Gossypium hirsutumL.) production. Previously, we identified and mapped the broad‐spectrum CBB‐resistant locusBB‐13on the long arm of chromosome D02 using array‐based single nucleotide polymorphisms (SNPs). In the current study, linked SNPs were converted into easily assayable Kompetitive Allele‐Specific PCR (KASP) markers to enable efficient detection and marker‐assisted selection of alleles at theBB‐13locus. The KASP marker's efficiency in detecting theBB‐13resistant gene was validated using an Upland cotton diversity panel of 72 accessions phenotyped withXcmrace 18. The KASP marker NCBB‐KASP4, derived from the CottonSNP63K array‐based marker i25755Gh that is closely associated withBB‐13, predicted the CBB response phenotypes with an error rate of 4.17% in the diversity panel. Additionally, two independent biparental recombinant inbred line populations segregating for resistance toXcmrace 18 were used for KASP marker validation and to test their utility in detecting the presence of theBB‐13locus in the resistant accession CABD3CABCH‐1‐89. NCBB‐KASP4, validated across breeding populations and broad germplasm, is a reliable KASP marker for detection and testing ofBB‐13locus in cotton. Further, diagnostic array‐based SNP marker i25755Gh's allele pattern and the potential CBB response is described for 875Gossypiumaccessions. These SNP‐based phenotypic predictions for 875 accessions along with disease response phenotypes toXcmrace 18 for 253 accessions provide a reference for CBB resistance in diverse cotton germplasm in the United States.